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Invitrogen™ MCP-1 Human ELISA Kit

For the quantitative detection of human MCP-1.

Brand:  Invitrogen™ BMS281TEN

27634.71 SEK valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.23750kg

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Product Code. 15571877

  • 41450.00 SEK / Pack of 10
Estimated Shipment: 10-04-2024
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Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human MCP-1
HRP-Conjugate anti-human MCP-1 monoclonal antibody
Human MCP-1 Standard lyophilized, 2ng/mL upon reconstitution
Control high, lyophilized
Control low, lyophilized
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

Description

Description

The Human Monocyte Chemoattractant Protein-1(Hu MCP-1) ELISA quantitates Hu MCP-1 in human serum, plasma,amniotic fluid, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MCP-1. Principle of the method The Human MCP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

MCP-1 (Monocyte chemotactic protein-1, CCL2) has a predicted molecular weight of approximately 11kDa, however, it may run at a higher molecular weight due to glycosylation. MCP-1 is a member of the cytokine family and plays an important role in immunoregulation and inflammatory processes. MCP-1 signals through G protein-coupled receptors, CCR2 and CCR4, to recruit memory T cells, monocytes and dendritic cells. MCP-1 is linked with tumor progression, angiogenesis, and displays selective chemotactic activity towards monocytes and basophils but not neutrophils or eosinophils. MCP-1 is produced by a variety of cell types and is a potent chemoattractant for monocytes, memory T lymphocytes, and natural killer (NK) cells. Further, MCP-1 is indicated in the etiology of several immune disorders, and is upregulated during infection and inflammation. While MCP-1 is a potent basophil activator, the related protein MCP-2 stimulates both eosinophils and basophils. MCP-3 has been shown to have the broadest range of influence. MCP-1 has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis.
Specifications

Specifications

P13500
2.3 pg/mL
ELISA Kit
Human
Colorimetric Microplate Reader
GDCF-2,HC11,HSMCR30,MCAF,MCP-1,MCP1,SCYA2,SMC-CF
4.7%
HRP
RUO
2°C to 8°C
Human
2 hr. 10 min.
15.6-1,000 pg/mL
HRP
Amniotic Fluid, Plasma, Serum, Supernatant
ELISA
6347
8.7%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
10 x 96 Tests
Amniotic Fluid, 20 μL; Plasma, 20 μL; Serum, 20 μL; Supernatant, 20 μL
Monocyte chemoattractant protein 1, MCAF, HC11
1 hr. 20 min.
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