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Invitrogen™ RANTES Human ELISA Kit
Measure levels of regulated upon activation, normal T-cell expressed and secreted (RANTES) in human serum, plasma, tissue extracts, urine and culture supernatant samples.
Brand: Invitrogen™ EHRNTS
Additional Details : Weight : 0.45000kg Transport : UN number : 3261 Chem class : 8 Pack group : III
Description
Assay RANTES (regulated upon activation, normal T-cell expressed and secreted) levels in human serum, plasma, tissue extracts, urine and culture supernatant samples with our Invitrgen™ Human RANTES ELISA Kits.
These human RANTES ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of RANTES (regulated upon activation, normal T-cell expressed and secreted) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.Target: Natural and recombinant human RANTES (regulated upon activation, normal T-cell expressed and secreted)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 51.2 to 2000pg/mL
Sensitivity: < 2pg/mL
Precision: Intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with human Eotaxin, GROα, GROβ, MIP-1α, MIP-1β, MCP-1, MCP-2, MCP-3, MCP-4, G-CSF, GM-CSF, IFNα, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-16, IL-17, TNFα or TNFβ, or rat RANTES
Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
Specifications
| P13501 | |
| 2 pg/mL | |
| Biotin | |
| ELISA Kit | |
| No significant cross-reaction with human Eotaxin, GROa, GROß, MIP-1a, MIP-1ß, MCP-1, MCP-2, MCP-3, MCP-4, G-CSF, GM-CSF, IFNa, IFNg, IL-1a, IL-1ß, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-16, IL-17, TNF | |
| Colorimetric Microplate Reader | |
| D17S136E,RANTES,SCYA5,SIS-delta,SISd,TCP228,eoCP | |
| <10% | |
| HRP | |
| Intra-assay CV <10%; inter-assay CV <10% | |
| RUO | |
| 2°C to 8°C | |
| 1 hr. 20 min. |
| 51.2 to 2000pg/mL | |
| Human RANTES ELISA Kit | |
| Colorimetric ELISA (TMB substrate) with Plates | |
| Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum, Urine | |
| 96 microplate wells | |
| 6352 | |
| <12% | |
| TMB Substrate Solution, 13mL, 30X Wash Buffer, 50mL, Streptavidin-HRP Concentrate, 75µL, Adhesive Plate Covers, 6 ea, Anti-Human RANTES Precoated 96-well Strip Plate, 1 ea, HRP Dilution Buffer, 14mL, Lyophilized Recombinant Human RANTES Standard, 2 vials, Standard Diluent, 2 x 25mL, Stop Solution, 13mL, Biotinylated Antibody Reagent, 8mL | |
| Absorbance at 450nm minus absorbance at 550nm | |
| 96 Tests | |
| Plasma,1μL; Serum,1μL; Supernatant,50μL; Urine,50μL | |
| Human | |
| 3 hr. |