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Invitrogen™ S100A8 Human ELISA Kit

Produktkod. 15415123
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Kvantitet:
96 Tests
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1 uppsättning
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Produktkod. 15415123

Brand: Invitrogen™ EHS100A8

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Beskrivning

Sandwich ELISA Kit

The Human S100A8 ELISA quantitates Hu S100A8 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu S100A8. Principle of the method The Human S100A8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.
TRUSTED_SUSTAINABILITY

Specifikationer

Tillträdesnummer P05109
Analysintervall 10.24-2500 pg/mL
Analyskänslighet 10 pg/mL
Kalibreringsområde 10pg/mL to 2500pg/mL
Analysera Solid-phase sandwich ELISA
Konjugera Biotin
Produkttyp ELISA Kit
Proteinfamilj Chemokine & Receptors
Provtyp Plasma, Serum, Supernatant
Specificitet This ELISA pair antibody detects human S-100A8. Other species not determined.
Tillräckligt för 96 assays
Målarter Human
För användning med (applikation) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
För användning med (utrustning) Colorimetric Microplate Reader
Gen-ID (Entrez) 6279
Gensymbol S100A8
Interassay CV <12%
Intraassay CV <10%
Kit Innehåll Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Etikett eller färgämne HRP
Kvantitet 96 Tests
Regulatorisk status RUO
Forskningsdisciplin Inflammation
Provvolym Plasma, 33 μL; Serum, 33 μL; Supernatant, 100 μL
Förvaringskrav 2°C to 8°C
Synonym 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8
Mål Human
Testtid 1 hr. 20 min.
Total analystid 4 hr. 45 min.
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