missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ eBioscience™ Human sAPO-1/Fas Platinum ELISA Kit

For the quantitative detection of human sAPO-1/Fas.

Brand:  Invitrogen™ BMS245

Additional Details : Weight : 0.23750kg

 View more versions of this product

Product Code. 15501827

  • 4930.00 SEK / 96 tests


EU Stock 2
Add to basket

Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sAPO-1/Fas
Biotin-Conjugate anti-human sAPO-1/Fas monoclonal antibody
Streptavidin-HRP
Human sAPO-1/Fas Standard lyophilized, 2ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

Description

Description

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.
Specifications

Specifications

P25445
Biotin
355
Pre-coated 96 well plate, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Controls
96 tests
13.2 pg/mL
10μL
Human
1 hr 20 min
Human
CD95, Fas receptor, APT 1, ALPS 1 A, TNFRSF6
4.5%
15.6-1,000 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA
RUO
Serum, Supernatants
Serum (10μL), Supernatants (10μL)
2-8°C
2 hr 10 min
FAS
3.1%
HRP
Videos

For Research Use Only