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Invitrogen™ CTLA-4 (Soluble) Human ELISA Kit

For the quantitative detection of human sCTLA-4.

Brand:  Invitrogen™ BMS276TEN

27634.71 SEK valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.23750kg

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Product Code. 15501867

  • 41450.00 SEK / Pack of 10
Estimated Shipment: 11-04-2024
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Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCTLA-4
Biotin-Conjugate anti-human sCTLA-4 monoclonal antibody
Streptavidin-HRP
Human sCTLA-4 Standard lyophilized, 20ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

Description

The Human Cytotoxic T Lymphocyte associated gene –4 (CTLA-4) ELISA quantitates Hu CTLA-4 in human serum, plasma, amniotic fluid, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CTLA-4.

Principle of the method The Human CTLA-4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.
Specifications

Specifications

P16410
0.13 ng/mL
ELISA Kit
Human
Colorimetric Microplate Reader
ALPS5,CD,CD152,CELIAC3,CTLA-4,GRD4,GSE,IDDM12
4.8%
HRP
RUO
2°C to 8°C
Human
3 hr. 10 min.
0.16-10.0 ng/mL
Biotin
Amniotic Fluid, Plasma, Serum, Supernatant
ELISA
1493
10.4%
Pre-coated 96 well plate, Standard, Standard, Assay Buffer concentrate, Assay Buffer concentrate, SAV-HRP, SAV-HRP, Chromogen, Chromogen, Stop Solution, Controls
10 x 96 Tests
Amniotic Fluid, 10 μL; Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL
Cytotoxic T-lymphocyte protein 4, ICOS, CD28
1 hr. 20 min.
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