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Invitrogen™ CD40L (Soluble) Human ELISA Kit
For the quantitative detection of human sCD40L.
6060.00 SEK - 41450.00 SEK
Specifications
Accession Number | P29965 |
---|---|
Assay Range | 0.16-10.0 ng/mL |
Assay Sensitivity | 0.06 ng/mL |
Conjugate | HRP |
Product Type | ELISA Kit |
Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD40L
HRP-Conjugate anti-human sCD40L monoclonal antibody
Human sCD40L Standard lyophilized, 20ng/mL upon reconstitution
Control high, lyophilized
Control low, lyophilized
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films
Dilution Plates
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15571797
|
Invitrogen™
BMS239 |
96 Tests |
6060.00 SEK
96 tests |
In Stock
Log in to see stock. |
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15521807
|
Invitrogen™
BMS239TEN |
10 x 96 Tests |
41450.00 SEK
Pack of 10 |
Estimated Shipment: 22-05-2024
Log in to see stock. |
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Description
The Human CD40 Ligand (Hu CD40L)) ELISA quantitates Hu CD40L in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD40L. Principle of the method The Human CD40L solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.Specifications
P29965 | |
0.06 ng/mL | |
ELISA Kit | |
Human | |
Colorimetric Microplate Reader | |
CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39,hCD40L | |
4% | |
HRP | |
Plasma, 20 μL; Serum, 20 μL; Supernatant, 20 μL | |
TRAP, HIGM 1, T cell antigen Gp39, CD154 | |
1 hr. 20 min. |
0.16-10.0 ng/mL | |
HRP | |
Plasma, Serum, Supernatant | |
ELISA | |
959 | |
6.8% | |
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers | |
RUO | |
2°C to 8°C | |
Human | |
2 hr. 10 min. |
For Research Use Only