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Invitrogen™ ICAM-1 (Soluble) Human ELISA Kit

For the quantitative detection of human sICAM-1.

6070.00 SEK - 41450.00 SEK

Specifications

Accession Number P05362
Assay Range 6.25-100 ng/mL
Assay Sensitivity 2.2 ng/mL
Conjugate HRP
Product Type ELISA Kit
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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sICAM-1
HRP Conjugate anti-human sICAM-1 monoclonal antibody
Human sICAM-1 Standard, 100ng/mL
Control high, lyophilized
Control low, lyophilized
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

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Product Code Brand Quantity Price Quantity & Availability  
15501487
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Invitrogen™
BMS201
96 Tests
6070.00 SEK
96 tests
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15541507
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Invitrogen™
BMS201TEN
10 x 96 Tests
41450.00 SEK
Pack of 10
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Estimated Shipment: 10-04-2024
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Description

Description

The Human Soluble Intercellular Adhesion Molecule 1(Hu sICAM-1) ELISA quantitates Hu sICAM-1 in human serum, plasma, cell culture supernatants, urine, amniotic fluid, bile, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sICAM-1. Principle of the method The Human sICAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.
Specifications

Specifications

P05362
2.2 ng/mL
ELISA Kit
Human
Colorimetric Microplate Reader
BB2,CD54,P3.58
4.1%
HRP
Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL
Intercellular adhesion molecule 1, CD54 antigen
1 hr. 20 min.
6.25-100 ng/mL
HRP
Plasma, Serum, Supernatant
ELISA
3383
7.7%
Pre-coated 96 well plates, Standard, Sample Diluent, Assay Buffer concentrate, Detection Antibody Conjugate: anti-rabbit-HRP, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
RUO
2°C to 8°C
Human
1 hr. 10 min.
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