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Applied Biosystems™ Power SYBR™ Green RNA-to-C_T™ 1-Step Kit

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

Brand: Applied Biosystems™ 4389986

5773.62 SEK valid until 2025-06-21
Use promo code "24112" to get your promotional price.

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Product Code. 10229194

8660.00 SEK / 200 reactions

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For Research Use Only. All usage must comply with product instructions.

Alert:

To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price

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Description

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction.
Formulated to ensure maximum sensitivity and reliability
Reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets

Includes:

AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation
ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA
An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals
RNase inhibitor, to prevent degradation of RNA templates
A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments

Order Info

Shipping Conditions: Wet Ice

Specifications

Content And Storage	Sufficient for 200 reactions at 50 μL reaction volume and includes: • 1 x 5 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1,and optimized buffer components. Store at 2-8°C after opening. • 1 x 80 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15 to -30°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
PCR Method	1-step RT-qPCR
Reaction Speed	Standard
Label or Dye	SYBR Green
Product Line	RNA-to-C_T™, SYBR™
Purity or Quality Grade	UP (Ultra Pure)
Reverse Transcriptase	ArrayScript™ UP
Shipping Condition	Wet Ice
For Use With (Application)	Gene Expression
Sample Type	RNA

Detection Method	SYBR
Polymerase	AmpliTaq Gold DNA Polymerase
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Passive Reference Dye	ROX (Pre-mixed)
Product Type	One-Step qRT-PCR Kit
Quantity	200 reactions
Run Time	Standard
Sufficient For	200 Reactions
No. of Reactions	200 Reactions

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Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit > 200 Reactions

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For Research Use Only. Not for use in diagnostic procedures.

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