IDO Rat anti-Mouse, PerCP-eFluor™ 710, Clone: mIDO-48, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 46-9473-80
Additional Details : Weight : 0.09500kg
Description: The monoclonal antibody mIDO-48 reacts with mouse indoleamine 2,3-dioxygenase 1(IDO1). IDO1 is an enzyme expressed intracellularly by dendritic cells, interferon gamma activated macrophages, epithelial cells, vascular endothelium and tumor cells. This enzyme catalyzes tryptophan into kynurenine metabolites. The reduction of tryptophan and the introduction of kynurenine metabolites in the microenvironment of IDO-expressing cells suppress T cell proliferation and promote the activation and induction of regulatory T cells. Applications Reported: This mIDO-48 antibody has been reported for use in flow cytometric analysis. Applications Tested: This mIDO-48 antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resource Tab online. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. Gamma-interferon (IFNG, MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO, EC 18.104.22.168). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.