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Invitrogen™ Chicken IFN gamma Matched Antibody Pair

Produktkod. 10738093
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Kvantitet:
10 x 96 Tests
Förpackningsstorlek:
Varje
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10738093 10 x 96 Tests Varje
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For Research Use Only. All usage must comply with product instructions.
Produktkod. 10738093 Leverantör Invitrogen™ Leverantörsnummer CAC1233
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Beräknad leverans: 07-08-2026
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For Research Use Only. All usage must comply with product instructions.

Beskrivning

ELISA

The Chicken Interferon gamma Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of chicken IFNγ. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
TRUSTED_SUSTAINABILITY

Specifikationer

Tillträdesnummer P49708
Analysintervall 15.6-1000 pg/mL
Analyskänslighet <15.6 pg/mL
Konjugera HRP
Genaccessionsnr. P49708
Gensymboler IFNG
Immunoassay Kit Format Matched Antibody Pair
Produkttyp ELISA, ELISA Kit
Kvantitet 10 x 96 Tests
Provtyp Plasma, Serum, Supernatant
Typ av substrat Adaptor Proteins
Gen-ID (Entrez) 396054
Målarter Chicken
För användning med (utrustning) Absorbance Microplate Reader, Colorimetric Microplate Reader
Gene Alias BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN γ; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFNγ; Immune interferon; Interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon γ; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-gBoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN γ; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFNγ; Immune interferon; Interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon γ; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-g;
Innehåll och lagring Antibody pair Kit contains matched, pre-titred and fully optimized Capture (coating) and Detection Antibodies, 3 vials of Recombinant Standard, Streptavidin-HRP concentrate and technical data sheet.
Store at 2-8°C.
Gensymbol IFNG
Kit Innehåll Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Etikett eller färgämne Biotin
Proteinaliaser IFN-gamma
Regulatorisk status RUO
Frakt skick Wet or Dry Ice
Förvaringskrav 2-8°C
Mål IFN gamma
Testtid 1 hr 30 min
Total analystid 24 hr
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