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Invitrogen™ IFN gamma Human ELISA Kit
For the quantitative detection of human IFN-gamma.
Brand: Invitrogen™ BMS228
Additional Details : Weight : 0.23750kg
Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human IFN-gamma
Biotin-Conjugate anti-human IFN-gamma monoclonal antibody
Streptavidin-HRP
Human IFN-gamma Standard lyophilized, 200ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
Description
IFN gamma (Interferon gamma,Type II interferon) is a macrophage activation factor,and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens,mitogens,Staphylococcus enterotoxin B,phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists,functionally,as a homodimer of approximately 45 kDa. On SDS-PAGE,IFN gamma appears as a combination of 25,20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity,tumor antiproliferative activity,induction of class I and II MHC,macrophage activation,and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II,and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2,FGF basic,EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.Specifications
P01579 | |
0.99pg/mL | |
ELISA Kit | |
Human | |
Colorimetric Microplate Reader | |
IFNG | |
4.5% | |
96 Tests | |
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL | |
Immune interferon, IFG | |
1 hr. 20 min. |
1.6-100pg/mL | |
Biotin | |
Plasma, Serum, Supernatant | |
ELISA | |
3458 | |
5.7% | |
HRP | |
RUO | |
2°C to 8°C | |
Human | |
3 hr. 10 min. |
For Research Use Only