IFN gamma, Functional Grade, clone: NIB42, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 16-7318-85
Additional Details : Weight : 0.01000kg
DescriptionDescription: The NIB42 antibody reacts with human interferon-gamma (IFN-gamma). The NIB42 antibody is a neutralizing antibody. IFN-gamma is an ∽20kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-gamma in synergy with other cytokines, such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity.Applications Reported: The NIB42 antibody has been reported for use in neutralization of human IFN gamma and for ELISA capture.Applications Tested: The Functional Grade Purified NIB42 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested for ELISA capture and in bioassay for neutralization of IFN gamma bioactivity. The NIB42 antibody at 5 μg/mL has been found to inhibit by 50% the biological effects of 1 ng/mL human IFN gamma(ND50), in an EMCV assay of A549 cell protection. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols™ section. The NIB42 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interferon-gamma (IFN-g) in combination with the biotin 4S.B3 (13-7319) antibody for detection and recombinant human IFNg (39-8319) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 2-8 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.For ELISPOT capture, the alternative clone MD-1 is recommended.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|ELISA, Functional Assay, Neutralization|
|IgG1, kappa, kappa|
For Research Use Only.