Mouse IgD, Functional Grade, clone: 10.4.22, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 16-5924-82
Additional Details : Weight : 0.09500kg
DescriptionDescription: This 10.4.22 monoclonal antibody reacts with the a allotype of mouse IgD, which is expressed by mature peripheral B cells. This antibody has been reported to activate B cells via engagement of the IgD receptor. The use of anti-IgM as a polyclonal B cell activator is problematic due to interference from high levels of circulating serum IgM.Crossblocking studies suggest that the 10.4.22 antibody recognizes a different epitope from 11-26c.Applications Reported: This 10.4.22 antibody has been reported for use in functional assays.Applications Tested: This 10.4.22 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|Store at 2-8°C.|
|Functional AssayþIn vitro Assay|
|Immunoglobulin D, Ig-D|
For Research Use Only.