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Invitrogen™ IgG (Total) Rat Uncoated ELISA Kit

For quantitative detection of rat total IgG.

4185.00 SEK - 6010.00 SEK

Specifications

For Use With (Application) ELISA
Synonym Immunoglobulin G
Target Rat
Sensitivity 1.6 ng/mL
Storage Requirements 2°C to 8°C
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Product Code Brand Quantity Price Quantity & Availability  
15580917
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Invitrogen™
88-50490-77
20 x 96 Tests
6010.00 SEK
Pack of 20
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Estimated Shipment
01-03-2021
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15500927
View Documents
Invitrogen™
88-50490-88
10 x 96 Tests
4185.00 SEK
Pack of 10
View alternative products
Save Up to 
»
Estimated Shipment
01-03-2021
Add to basket
 
Description

Description

The Rat Total Immunoglobulin G (IgG) Uncoated ELISA Kit contains pre-matched antibody pairs, plates, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of rat total IgG. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Specifications

Specifications

ELISA
Rat
2°C to 8°C
Immunoglobulin G
1.6 ng/mL
Rat
SDS

For Research Use Only