Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™, Invitrogen™

Description

Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies. Applications Reported: This mouse IgG2a isotype control has been reported for use in cell surface and intracellular flow cytometric analysis, immunocytochemistry, and immunohistochemistry. Applications Tested: The FITC conjugated mouse IgG2a isotype control is offered in 2 formats: - μg size: has been tested by flow cytometric analysis of normal human peripheral blood cells and should be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at test size: 5 μL 1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.