Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™, Invitrogen™

Description

Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin. Applications Reported: This rat IgG2a isotype control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis. Applications Tested: The FITC conjugated rat IgG2a isotype control is offered in 2 formats: - μg size: This rat IgG2a isotype control has been tested by flow cytometric analysis of mouse splenocytes. This can be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells and can be used at 5 μL for the catalog number ending in -41 or -42 and 20μL/test for the product ending in -73 per test. Each test size contains 0.5 μg. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains. It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.