Mouse anti-Rat IgG2a, eFluor 660, clone: r2a-21B2, Secondary Antibody, eBioscience™
Mouse Monoclonal Secondary Antibody
Brand: Affymetrix eBioscience 50-4817-82
Additional Details : Weight : 0.09500kg
DescriptionDescription: The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2a or any mouse isotype antibodies.Applications Reported: This r2a-21B2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This r2a-21B2 antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells stained with a rat IgG2a primary antibody. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.