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Rat anti-Mouse IgG2a Secondary Antibody, eFluor™ 570, eBioscience™, Invitrogen™
Rat Monoclonal Secondary Antibody
Brand: Affymetrix eBioscience 41-4210-80
Additional Details : Weight : 0.25000kg
Description
Description: The monoclonal antibody m2a-15F8 recognizes Mouse IgG2a antibodies and can be used as a second step reagent in microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG2a or any rat isotype antibodies. Applications Reported: This m2a-15F8 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunohistochemical staining, and immunocytochemistry. Applications Tested: This m2a-15F8 antibody has been tested immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG2a isotype. This can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor™ 555 or TRITC filter is acceptable. Excitation: 555 nm; Emission: 570 nm
Mouse IgG2a Monoclonal antibody specifically detects Mouse IgG2a in Mouse samples. It is validated for Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin).Specifications
Mouse IgG2a | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
Affinity chromatography | |
RUO | |
Mouse | |
Liquid |
Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) | |
m2a-15F8 | |
eFluor 570 | |
Rat | |
25 μg | |
Secondary | |
4° C, store in dark, DO NOT FREEZE! | |
IgG1 κ |
For Research Use Only.