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IgM Monoclonal Antibody (eB121-15F9), PE, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

906.00 SEK - 2110.00 SEK

Specifications

Antigen IgM
Clone eB121-15F9
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15278879
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Affymetrix eBioscience
12-5890-82
100 μg
2110.00 SEK
100µg
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15268879
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Affymetrix eBioscience
12-5890-81
50 μg This item is currently unavailable or has been discontinued.
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Description

Description

Description: The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM. Applications Reported: eB121-15F9 has been reported for use in flow cytometric analysis to detect the surface IgM on mouse B cells and as a second step to detect primary mouse IgMs. Applications Tested: The eB121-15F9 antibody has been tested by flow cytometric analysis to detect the surface IgM on mouse B cells and can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.
Specifications

Specifications

IgM
0.2 mg/mL
Monoclonal
Liquid
RUO
16019
IgG2a κ
Affinity chromatography
Antibody
eB121-15F9
Flow Cytometry
PE
Rat
Mouse
Ighm
Primary
4° C, store in dark, DO NOT FREEZE!
Ighm
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SDS
Documents

Documents

Product Certifications

For Research Use Only.