Mouse IgM, PE, clone: eB121-15F9, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 12-5890-81
Additional Details : Weight : 0.25000kg
DescriptionDescription: The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM.Applications Reported: eB121-15F9 has been reported for use in flow cytometric analysis to detect the surface IgM on mouse B cells and as a second step to detect primary mouse IgMs.Applications Tested: The eB121-15F9 antibody has been tested by flow cytometric analysis to detect the surface IgM on mouse B cells and can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|Immunoglobulin M, Ig M|
For Research Use Only.