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IL-1 alpha, clone: ALF-161, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-7011-81

Additional Details : Weight : 0.01000kg

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Product Code. 15237977

  • 752.00 SEK / 50µg


Estimated Shipment date
from Supplier 21-11-2018
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Description

Description

Description: The ALF-161 antibody reacts with mouse interleukin-1alpha (IL-1alpha). Mouse IL-1alpha, also called Lymphocyte Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), and Mononuclear Cell Factor (MCF), is a 17kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1alpha is mostly cell-associated, with 23% amino acid homology with IL-1beta. The immune regulatory role of IL-1alpha is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.Applications Reported: The ALF-161 antibody has been reported for use in ELISA capture.Applications Tested: The ALF-161 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-1 alpha (IL-1 alpha) in combination with the biotin anti-mouse Interleukin-1 alpha (IL-1a) polyclonal (13-7111) antibody for detection and recombinant mouse IL-1a (14-8011) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-1 alpha
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P01582
Interleukin-1 alpha, Hematopoietin-1, IL1A
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
ELISA
ALF-161
Unconjugated
Il1a
Liquid
Il1a
IgG
50μg
Store at 2-8°C.
Primary
16175
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