missing translation for 'onlineSavingsMsg'
Learn More

IL-1 alpha Mouse anti-Human, FITC, Clone: 364/3B3-14, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-7118-82

Additional Details : Weight : 0.25000kg

 View more versions of this product

Product Code. 15238359

  • 2205.00 SEK / 100µg

Estimated Shipment: 02-07-2021

Please to purchase this item. Need a web account? Register with us today!


Description

Description

Description: The 364/3B3-14 antibody reacts with human interleukin-1alpha. Applications Reported: The 364/3B3-14 antibody has been reported for use as capture antibody in a human IL-1 alpha ELISA and for intracellular staining for flow cytometric analysis. Applications Tested: This 364/3B3-14 antibody is offered in 2 formats: - µg size: has been tested by intracellular staining and flow cytometric analysis. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Specifications

Specifications

IL-1 alpha
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P01583
Interleukin-1 alpha, Hematopoietin-1, IL1A
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
364/3B3-14
FITC
IL1A
Liquid
IL1A
IgG1
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
3552
Videos
SDS
Documents
Certificates

For Research Use Only.