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Invitrogen™ Human IL-10 Matched Antibody Pair

Produktkod. 10775153
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Kvantitet:
10 x 96 Tests
Förpackningsstorlek:
Varje
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For Research Use Only. All usage must comply with product instructions.

Produktkod. 10775153 Leverantör Invitrogen™ Leverantörsnummer CHC1323
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Beräknad leverans: 07-08-2026
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For Research Use Only. All usage must comply with product instructions.

ELISA

The Human IL-10 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-10. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
TRUSTED_SUSTAINABILITY

Specifications

Tillträdesnummer P22301
Analysintervall 31.2-2000 pg/mL
Analyskänslighet 31.2 pg/mL
Konjugera HRP
Genaccessionsnr. P22301
Gene Alias CSIF, GVHDS, IL-10, IL10A, MGC126450, MGC126451, TGIF, CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIFCSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF; CSIF; GVHDS; IL-10A; TGIF; IL10
Gensymboler IL10
Immunoassay Kit Format Matched Antibody Pair
Produkttyp ELISA, ELISA Kit
Kvantitet 10 x 96 Tests
Provtyp Plasma, Serum, Supernatant
Gen-ID (Entrez) 3586
Målarter Human
För användning med (utrustning) Absorbance Microplate Reader, Colorimetric Microplate Reader
Inkluderar Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Innehåll och lagring Antibody pair Kit contains matched, pre-titred and fully optimized Capture (coating) and Detection Antibodies, 3 vials of Recombinant Standard, Streptavidin-HRP concentrate and technical data sheet.
Store at 2-8°C.
Gensymbol IL10
Kit Innehåll Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Etikett eller färgämne Biotin
Proteinaliaser IL-10, Cytokine synthesis inhibitory factor, CSIF
Regulatorisk status RUO
Frakt skick Wet Ice
Förvaringskrav 2-8°C
Mål IL-10
Testtid 1 hr 30 min
Total analystid 24 hr
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