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IL-10, PE, clone: JES5-16E3, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-7101-81

Additional Details : Weight : 0.25000kg

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Product Code. 15239289

  • 1040.00 SEK / 50µg


Estimated Shipment date
from Supplier 28-11-2018
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Description

Description

Description: The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). Mouse IL-10 is an ∽18kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.Applications Reported: The JES5-16E3 antibody has been reported for use in capture of mouse IL-10 by ELISA and ELISPOT, intracellular staining for flow cytometric analysis, IHC, and neutralization of IL-10 bioactivity.Applications Tested: This JES5-16E3 antibody is tested by intracellular staining and flow cytometric analysis of cultured mouse splenocytes. It is offered in 2 formats: -μg size: has been tested by intracellular staining and flow cytometric analysis of cultured mouse splenocytes. This can be used at less than or equal to 0.25μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of cultured mouse splenocytes. This can be used at 5μL (0.125μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-10
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P18893
Interleukin-10, CSIF, IL-10, IL10A, TGIF
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
JES5-16E3
PE
Il10
Liquid
Il10
IgG2b, kappa, kappa
50μg
4° C, store in dark, DO NOT FREEZE!
Primary
16153
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