IL-12/IL-23 p40, Functional Grade, clone: C8.6, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 16-7129-85
Additional Details : Weight : 0.01000kg
DescriptionDescription: The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.Applications Reported: The C8.6 antibody has been reported for use ELISA, ELISPOTand neutralization.Applications Tested: The Functional Grade Purified C8.6 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested for ELISA detection and in bioassay for neutralization of IL-12 p70 and IL-23 bioactivity. The C8.6 antibody at 60.0 ng/mL has been found to inhibit by 50% the biological effects of 1 ng/mL human IL-12 p70 (14-8129) or human IL-23 (14-8239), in corresponding bioassay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|IL12B, Il12p40, interleukin 12B, RP23-388G23.1, Il-12b, Il-12p40, p40, IL-12 p40, IL-23 subunit p40, IL12P40|
|Cytokine NeutralizationþELISAþDot blot|
|Store at 2-8°C.|
For Research Use Only.