IL-12 p35 Mouse anti-Mouse, Functional Grade, Clone: MMp35A1.6 (A1.6), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 16-7360-82
Additional Details : Weight : 0.09500kg
DescriptionMore recently, p35 was also found to dimerize with EBI3 (Epstein-Barr Virus-Induced Gene 3) to form IL-35. The biology of IL-35 is incompletely understood, and it is believed to be unique among the IL-12 family members in its expression and function. It is produced by regulatory T cells, and appears to have immunoregulatory functions. IL-35 has been found to suppress the proliferation and function of effector T cells, and has also been observed to induce a regulatory phenotype on naive CD4+ cells cultured in its presence. Applications Reported: This MMp35A1.6 monoclonal antibody has been reported for use in functional assays. Applications Tested: This MMp35A1.6 antibody at less than or equal to 20 µg/mL has been found to inhibit by 50% the biological effects of 1 ng/mL Mouse IL-12 p70 Recombit Protein (14-8121), as measured by neutralization of mouse IFN gamma induction in normal mouse splenocytes. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC. Interleukin 12A is a novel signaling cytokine belonging to the Interleukin-6 protein superfamily. Interleukin-12 alpha chain precursor (IL-12A) is a 35-kD subunit of disulfide-linked heterodimer of cytokine IL-12 and has a broad array of biological activities. Reports suggest that IL-12A can act as a growth factor for activated T and NK cells and enhance the lytic activity of NK/lymphokine-activated Killer cells. IL-12A stimulates the production of IFN-gamma by resting PBMC as well as T-cell-independent induction of interferon (IFN)-gamma, and helps in the differentiation of both Th1 and Th2 cells. It is also involved in positive regulation of tyrosine phosphorylation of Stat4 protein. The signaling process of IL-12A in innate immunity requires nitric oxide synthase 2A. Along with EBI3, IL-12A forms a novel heterodimeric cytokine, designated as interleukin-35 (IL-35), that may be specifically produced by T reg cells and is required for maximal suppressive activity.
|PBS with no preservative; pH 7.2|
|CLMF, IL-12A, NFSK, NKSF1, P35, IL12p35|
For Research Use Only.