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IL-17A Rat anti-Mouse, Rat, FITC, Clone: eBio17B7, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-7177-80

Additional Details : Weight : 0.25000kg

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Product Code. 15248359

  • 868.00 SEK / 25µg


EU Stock 5
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Description

Description

The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN gamma and IL-4, have each been found to negatively regulate the generation of these Th-17 cells. Applications Reported: This eBio17B7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This eBio17B7 antibody has been tested by intracellular staining and flow cytometric analysis of restimulated, Th17-polarized splenocytes using the intracellular Fixation and Permeabilization Buffer Set (cat. 88-8824) and protocol. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Staining has been successfully done using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).
Specifications

Specifications

IL-17A
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q62386, Q61453
Interleukin-17
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse, Rat
Flow Cytometry, Immunohistochemistry (Paraffin)
eBio17B7
FITC
Il17a
Liquid
Il17a
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
16171, 301289
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