IL-19 Monoclonal Antibody (RN19), eBioscience™, Invitrogen™

Description

Description: The RN19 antibody reacts with mouse interleukin-19 (IL-19), a member of the IL-10 family, which is secreted by activated keratinocytes and monocytes. Applications Reported: The RN19 antibody has been reported for use in ELISA capture of mouse IL-19. Applications Tested: The RN19 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-19 (IL-19) in combination with a biotinylated polyclonal anti-mouse IL-19 antibody for detection. A suitable range of concentrations of this antibody for ELISA capture is 1.0-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/mL - 30 pg/mL should be included in each ELISA plate. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

The protein encoded by this gene is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Alternatively spliced transcript variants encoding the distinct isoforms have been described.