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Invitrogen™ IL-2 Human Uncoated ELISA Kit

Uncoated ELISA Kit

4915.00 SEK - 7060.00 SEK

Specifications

Accession Number P60568
Assay Range 2-250 pg/mL
Assay Sensitivity 2 pg/mL
Conjugate Biotin
Product Type ELISA Kit
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15570997
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Invitrogen™
88-7025-77
20 x 96 Tests
7060.00 SEK
Pack of 20
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15590997
View Documents Promotion Details
Invitrogen™
88-7025-88
10 x 96 Tests
4915.00 SEK
Pack of 10
View alternative products
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In Stock
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Description

Description

The Human Interleukin-2 (IL-2) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of Humanmouse IL-2. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
Specifications

Specifications

P60568
2 pg/mL
ELISA Kit
Human
Colorimetric Microplate Reader
Il-1a
HRP
Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
Interleukin-2
1 hr. 45 min.
2-250 pg/mL
Biotin
Plasma, Serum, Supernatant
ELISA
3558
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86)
RUO
2°C to 8°C
Human
24 hr. 30 min.
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