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IL-23 p19, clone: G23-8, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-7232-81

Additional Details : Weight : 0.01000kg

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Product Code. 15238047

  • 954.00 SEK / 50µg


Estimated Shipment date
from Supplier 27-03-2019
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Description

Description

Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-g production by naive and memory T cells, as compared to IL-12. Applications Reported: The G23-8 antibody has been reported for use as the capture antibody in mouse IL-23 ELISA, for Western blotting, and for neutralization of mouse IL-23 bioactivity. Applications Tested: The G23-8 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IL-23 (p19p40) protein levels in combination with the biotinylated (p40-specific) C17.8 antibody (13-7123) for detection and recombit mouse IL-23 (14-8231) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombit standard over the range of 4000 pg/mL - 30 pg/mL should be included in each ELISA plate. Important Note: TMB, rather than ABTS, should be used as a substrate to achieve this sensitivity level. For specific neutralization of mouse IL-23 protein activity (with no effect on IL-12 p70), the functional grade purified G23-8 antibody is recommended (cat No.16-7232). Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered. The p19 shares sequence similarity with IL-6 subfamily members and is distantly related to the p35 subunit of IL-12. It shows no biological activity by itself; instead, it combines with the p40 subunit of IL-12 to form a biologically active, composite cytokine, IL-23. IL-23 shares some in vivo functions with IL-12, including the activation of the transcription factor Stat4. Similar to IL-12, human IL-23 stimulates IFN-g production and proliferation of PHA blast T cells, as well as in CD45 RO (memory) T cells. Ubiquitous transgenic expression of the IL-23 subunit p19 induces multiorgan inflammation, runting, infertility and premature death.
Specifications

Specifications

IL-23 p19
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q9EQ14
IL-23, IL-23A, IL23P19, MGC79388, P19, SGRF
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
ELISA, Western Blot
G23-8
Unconjugated
Il23a
Liquid
Il23a
IgG1, kappa
50 μg
4° C
Primary
83430
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