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Invitrogen™ Human IL-6 ELISA Kit

Produktkod. 10390964
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Kvantitet:
96 tester
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1 uppsättning
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Produktkod. 10390964 Leverantör Invitrogen™ Leverantörsnummer EH2IL6
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ELISA

The Human Interleukin-6 (Hu IL-6) ELISA quantitates Hu IL-6 in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-6. Principle of the method The Human IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
TRUSTED_SUSTAINABILITY

Specifikationer

Tillträdesnummer P05231
Analysintervall 10,24 till 400 pg/ml
Analyskänslighet <1 pg/mL
Innehåll och lagring Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Beskrivning Humant IL-6 ELISA Utrustning
Konjugera HRP
Immunoassay Kit Format Kolorimetrisk ELISA (TMB-substrat) med plattor
Produkttyp ELISA
Proteinfamilj Cytokines & Receptors
Provtyp Plasma, Serum, Supernatant, Urin
Specificitet Ingen signifikant korsreaktion med humant IL-1 A, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFa, IFNg, IFNa, GM-CSF eller mus IL-6
Tillräckligt för 96 mikroplattor brunnar
Målarter Människa
För användning med (utrustning) Kolorimetrisk mikroplattläsare
Gene Alias B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6; BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6
Gen-ID (Entrez) 3569
Gensymbol BSF-2,BSF2,CDF,HGF,HSF,IFN-beta-2,IFNB2,IL-6
Interassay CV <10%
Intraassay CV <10%
Kit Innehåll Förbelagd 96 brunnars platta, Standard, Analysspädningskoncentrat, Biotinylerad detektionsantikropp, SAV-HRP, Tvättbuffert, Kromogen, Stoppa lösning, Självhäftande plåtskydd
Etikett eller färgämne Biotin
Mått Absorbans vid 450 nm minus absorbans (vid 550 nm)
Precision Intra-analys CV < 10%; inter-analys CV < 10 %
Kvantitet 96 tester
Regulatorisk status RUO
Provvolym Plasma 50μ L, Serum 50μ L, Supernatant 50μ L, Urin 50μ L
Förvaringskrav 2 °C till 8 °C
Mål IL-6
Testtid 1 tim 20 min
Total analystid 3 hr
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Produktbeteckning
  • Human IL-6 ELISA Kit
Signalord
  • Varning
Riskkategorier
  • Preobcutljivost koze Kategorija 1
Faroangivelser
  • H317-Kan orsaka allergisk hudreaktion.
Skyddsangivelse
  • P261-Undvik att inandas damm/rök/gaser/dimma/ångor/sprej.
  • P272-Nedstänkta arbetskläder får inte avlägsnas från arbetsplatsen.
  • P280-Använd skyddshandskar/skyddskläder/ögonskydd/ansiktsskydd.
  • P302+P352-VID HUDKONTAKT: Tvätta med mycket vatten/.
  • P333+P313-Vid hudirritation eller utslag: Sök läkarhjälp.
  • P362+P364-Ta av nedstänkta kläder och tvätta dem innan de används igen.
  • P501b-Innehållet/behållaren lämnas till I enlighet med lokala / regionala / nationella / internationella bestämmelser (ska anges).
Kompletterande information
  • MIXTURE LIST-Contain: 2-methyl-3(2H)-isothiazolone
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