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Invitrogen™ Human IL-6 Matched Antibody Pair

Produktkod. 10353493
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Kvantitet:
10 x 96 Tests
Förpackningsstorlek:
Varje
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10353493 10 x 96 Tests Varje
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For Research Use Only. All usage must comply with product instructions.
Produktkod. 10353493 Leverantör Invitrogen™ Leverantörsnummer CHC1263
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For Research Use Only. All usage must comply with product instructions.

Beskrivning

ELISA

The Human IL-6 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-6. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
TRUSTED_SUSTAINABILITY

Specifikationer

Tillträdesnummer P05231
Analysintervall 15.6-1000 pg/mL
Analyskänslighet 15.6 pg/mL
Konjugera HRP
Genaccessionsnr. P05231
Gene Alias BSF2, HGF, HSF, IFNB2, IL-6, B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6; BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6
Gensymboler IL6
Immunoassay Kit Format Matched Antibody Pair
Produkttyp ELISA, ELISA Kit
Kvantitet 10 x 96 Tests
Provtyp Plasma, Serum, Supernatant
Gen-ID (Entrez) 3569
Målarter Human
För användning med (utrustning) Absorbance Microplate Reader, Colorimetric Microplate Reader
Inkluderar Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Innehåll och lagring Antibody pair Kit contains matched, pre-titred and fully optimized Capture (coating) and Detection Antibodies, 3 vials of Recombinant Standard, Streptavidin-HRP concentrate and technical data sheet.
Store at 2-8°C.
Gensymbol Il6
Kit Innehåll Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Etikett eller färgämne Biotin
Regulatorisk status RUO
Frakt skick Wet Ice
Förvaringskrav 2-8°C
Mål IL-6
Testtid 1 hr 30 min
Total analystid 24 hr
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