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Invitrogen™ IL-7 Human ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EHIL7X5
Additional Details : Weight : 0.33820kg
Description
The Human Interleukin 7 (Hu IL-7) ELISA quantitates Hu IL-7 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-7. Principle of the method The Human IL-7 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.Specifications
P13232 | |
5 pg/mL | |
Solid-phase sandwich ELISA | |
ELISA Kit | |
Plasma, Serum, Supernatant | |
5 x 96 assays | |
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
3574 | |
<12% | |
Pre-coated 96 well plates, Biotinylated Detection Antibody, Stop Solution, Chromogen, SAV-HRP, Standard, Wash Buffer, Assay Diluent Concentrate, Adhesive Plate Covers | |
5 x 96 Tests | |
Immunology | |
2°C to 8°C | |
Human | |
4 hr. 45 min. |
4.1 pg/mL to 1000 pg/mL | |
5 pg/mL to 1000 pg/mL | |
Biotin | |
Other Proteins | |
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. | |
Human | |
Colorimetric Microplate Reader | |
IL-7 | |
<10% | |
HRP | |
RUO | |
Plasma, 50 μ;L; Serum, 50 μ;L; Supernatant, 100 μ;L | |
IL-7 | |
1 hr. 20 min. |