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STEMCELL Technologies IntestiCult™
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Kvantitet:
1 Kit
Förpackningsstorlek:
1 uppsättning
Beskrivning
- IntestiCult™ Organoid Differentiation Medium (Human) is a complete culture medium that supports the further differentiation of intestinal organoids in three dimensions (3D), or in 2D as monolayers or air-liquid interface (ALI) cultures
- The starting cultures can be either intestinal organoids derived from human intestinal crypts, or passaged organoids that have been cultured with IntestiCult™ Organoid Growth Medium (Human; Catalog #06010)
- Intestinal cultures generated using IntestiCult™ Organoid Differentiation Medium (Human) contain physiologically relevant proportions of differentiated and stem cell populations, recapitulating the diversity of the crypt-villus axis
- When compared to conventional cell lines, intestinal monolayers exhibit greater barrier integrity, express higher levels of key differentiation markers, and have a morphology that is more representative of the in vivo intestine
- Applications of intestinal organoid cultures include studying the development and function of the intestinal epithelium, modeling intestinal diseases, compound screening, and regenerative therapy approaches
- Intestinal monolayer and ALI cultures are particularly amenable for permeability assays and studies of infectious diseases due to easy access to the apical surface
- This kit requires IntestiCult™ Organoid Growth Medium (Human; Catalog #06010) for the initiation and expansion of intestinal organoids prior to differentiation
Specifikationer
Specifikationer
| Celltyp | Intestinal Cells |
| För användning med (applikation) | Cell Culture; Differentiation; Expansion; Maintenance; Organoid Culture |
| Kvantitet | 1 Kit |
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