missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ Luria Broth Base (Miller's LB Broth Base), powder

Luria broth (LB) is a popular, rich medium used to cultivate bacteria and to perform coliphage plaque assays.

Brand:  Invitrogen™ 12795027

765.37 SEK valid until 2024-12-14
Use promo code "21632" to get your promotional price.


 View more versions of this product

Product Code. 11548866

  • 1148.00 SEK / 500g
Estimated Shipment: 16-12-2024
to see stock.
Explore more special offers
Add to basket

For Research Use Only. All usage must comply with product instructions.

Alert:

To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”21632” to get your promotional price

This item is not returnable. View return policy

Description

Description

Luria broth (LB) is a popular, rich medium used to cultivate bacteria and to perform coliphage plaque assays. The original formulation published in 1951 by Guiseppe Bertani to study phage plaque formation and named Lysogeny broth. It is also known as Lennox broth and Luria-Bertani broth.

Widely used LB medium formulations differ in salt concentration:
Miller: 10 g/L NaCl
Lennox: 5 g/L NaCl
Luria: 0.5 g/L NaCl

Luria Broth Base (Miller's formulation) is recommended for the general maintenance and growth of E. coli as it allows for fast cell expansion and supports high cell density. In cases where salt-sensitive antibiotics are being used for selection (such as Zeocin, tetracycline or chloramphenicol), Lennox formulation medium would be recommended.

Luria Broth Base formulation
Formulation per liter: 10 g SELECT Peptone 140, 5 g SELECT Yeast Extract, and 10 g NaCl.
To prepare 1 L of Miller-LB media, add 25 g of powder to 1 L distilled water and mix by vortexing. Adjust the pH to 7.0 with 5 N NaOH and sterilize by autoclaving for 25 minutes at 121°C.

Find the media format that fits your needs
We offer other ready-to-use media formulations, such as LB Broth (Cat. No. 10855001), Terrific Broth (Cat. No. A1374301) or S.O.C recovery medium (Cat. No. 15544034).

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Escherichia coli
Bottle
Autoclave
Coliforms
Room Temperature
• 500 g LB Dehydrated Media Powder

Store at room temperature.

LB Broth Base (Miller's LB Broth Base)
Dehydrated Culture Media
500 g
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.