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  7. Invitrogen™ Platinum™ Taq DNA Polymerase 

Invitrogen™ Platinum™ Taq DNA Polymerase

Code produit 10277202
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For Research Use Only. All usage must comply with product instructions.
Code produit 10277202 Fournisseur Invitrogen™ Code fournisseur 10966026
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For Research Use Only. All usage must comply with product instructions.

Description

Includes

Each box contains: Platinum Taq DNA Polymerase; 10X PCR Buffer (no magnesium chloride); 50mM magnesium chloride; KB Extender

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable hot-start thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The hot start property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step, thus preventing the extention of nonspecifically annealed primers and improving product yield.

The hot-start property allows for convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity. PCR products generated with Platinum Taq DNA Polymerase may be used in the same downstream applications without protocol modifications.

Features

  • Convenient reaction assembly at room temperature
  • 5' to 3' exonuclease activity
  • Lacks 3' to 5' exonuclease activity

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • Hot-start PCR
  • RT-PCR

Notes

  • For superior PCR performance, the next-generation enzyme Platinum II Taq Hot-start DNA Polymerase is recommended.
  • Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.

Order Info

Shipping Condition: Approved for shipment on wet or dry ice

TRUSTED_SUSTAINABILITY

Spécification

Content And Storage • Platinum Taq DNA Polymerase, 60 μL
• 10X PCR Buffer (without magnesium), 2 x 1.25 mL
• 50 mM MgCl2, 1 mL
• KB Extender, 1.25 mL

Store at -10°C to -30°C in a non-frost-free freezer.
Format Tube
GC-Rich PCR Performance Low
Polymerase Platinum Taq DNA Polymerase
Reaction Speed Standard
Exonuclease Activity 5' - 3'
Product Type DNA Polymerase
Quantity 300 reactions
Shipping Condition Wet or Dry Ice
For Use With (Application) Hot-start PCR
Fidelity (vs. Taq) 1X
Hot Start Built-In Hot Start
No. of Reactions 300 Reactions
Overhang 3'-A
Reaction Format Separate Components
Size (Final Product) 5 kb or less
Starting Material DNA
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For Research Use Only. Not for use in diagnostic procedures.

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