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Invitrogen™ PureLink™ HiPure Plasmid Midiprep Kit

The PureLink HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli.

Brand:  Invitrogen™ K210004

2111.64 SEK valid until 2024-05-31
Use promo code "21664" to get your promotional price.



Additional Details : CAS Number : 9048-46-8 Weight : 2.00000kg


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Product Code. 10083762

  • 2255.00 SEK / 1191mL
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For Research Use Only. All usage must comply with product instructions.


Description

Description

The PureLink HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Midiprep Kit protocol will typically yield 100–350 μg plasmid DNA from 15–25 mL bacterial culture, at purity that is comparable to that achieved by two passes through cesium chloride gradients.

  • Labor and time saving- no need for extra steps to remove endotoxin and other contaminants
  • High-purity, low-endotoxin product- pure enough for mammalian cell transfection
  • Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs

Anion-exchange chromatography for plasmid purification

  • PureLink HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to level equivalent to two passes through CsCl gradients
  • The resin combines excellent capacity with fast flow rate, high resolution, high yield, and efficient endotoxin removal
  • Plasmid preparations can typically be completed in less than 2 hours

Contaminant-free DNA

  • Unlike cesium chloride gradient protocols, PureLink HiPure Plasmid Purification Systems do not use troublesome solvents to work with and to dispose of
  • Typically, plasmid DNA prepared using the PureLink HiPure Plasmid Midiprep Kit will have an A260/A280 ratio of greater than 1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications
  • Endotoxin levels are typically within 0.1–1 EU/μg, making this plasmid DNA ideal for mammalian cell transfection
  • Purification time is two hours
Specifications

Specifications

• 100 mL Resuspension Buffer (R3)
• 550 μL RNase A
• 100 mL Lysis Buffer (L7)
• 100 mL Precipitation Buffer (N3)
• 250 mL Equilibration Buffer (EQ1)
• 500 mL Wash Buffer (W8)
• 125 mL Elution Buffer (E4)
• 15 mL TE Buffer
• 25 HiPure Columns
• 5 Column Holders

Store all components at room temperature.
Anion Exchange Resin
5 mL
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
25 Preps
100-200 μg (Medium-Scale) Plasmid DNA
Plasmid MidiPrep Kit
Transfection Grade
BAC DNA, Plasmid DNA
2 hr.
Column
Bacterial Culture
BAC DNA, Plasmid DNA
Not High-throughput Compatible (Manual)
<40kb, Low Copy Plasmid, High Copy Plasmid, BAC
PureLink™
25 Preps
Room Temperature
PureLink™
350 μg
Product Suggestions

Product Suggestions

Videos
Safety and Handling

Safety and Handling

Product Identifier
  • PureLink HiPure Plasmid Midiprep Kit

Signal Word
  • Warning

Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2

Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.

Precautionary Statement
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P362+P364-Take off contaminated clothing and wash it before reuse.

Supplemental information
  • MIXTURE LIST-Contains : acetic acid, Sodiumlauryl sulfate, Sodium hydroxide

SDS
Documents

Documents

Certificates
Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.