missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ PureLink™ HiPure Plasmid Miniprep Kit

Highly-quality purified plasmid DNA in under two hours

Brand:  Invitrogen™ K210002

Additional Details : CAS Number : 9048-46-8 Weight : 0.50000kg Transport : UN number : 1824 Chem class : 8 Pack group : III

 View more versions of this product

Product Code. 10013552

  • 1496.00 SEK / Each


In stock, ready to ship: 31
Add to basket

For Research Use Only. All usage must comply with product instructions.


Description

Description

The PureLink HiPure Plasmid Miniprep Kit allows isolation of high yields of highly pure plasmid DNA from E.coli in less than 2 hours, without use of any organic solvents or cesium chloride, and contains Low endotoxin levels.

Plasmid DNA of High Purity

  • PureLink HiPure Plasmid Miniprep Kit is designed to efficiently isolate plasmid DNA from E.coli in 1.5 - 2 hours
  • Kit employs anion-exchange resin composed of small particles with a uniform pore size to purify plasmid DNA to a level equivalent to two passes through CsCl gradients
  • Need for additional steps to remove contaminants such as phenol, chloroform, and CsCl are eliminated, minimizing exposure to and disposal of hazardous materials
  • Efficient – Increased yields with low endotoxin Levels
High Yield and Efficient Endotoxin Removal
  • PureLink HiPure Plasmid Miniprep Kit allows purification of up to 30μg of high quality plasmid DNA from 1-3mL overnight E.coli cultures in approximately 1 hour, with an endotoxin level of 0.1-1 EU/μg, when cloning high copy number plasmids
  • Typically, DNA isolated using the PureLink HiPure Plasmid Miniprep Kit has an A260/A280 >1.80 when samples are diluted in Tris-HCl (pH 7.5) indicating that the DNA is reasonably free of proteins that could interfere with downstream applications
Isolated DNA can be used for a Number of Downstream Applications:
  • Purified DNA is suitable for downstream applications, including those requiring the highest purity DNA, such as mammalian transfections, automated and manual DNA sequencing, PCR amplification, in vitro transcription, bacterial cell transformation, cloning, and labeling

DNA & RNA Purification & Analysis, DNA Extraction, Miniprep, Plasmid DNA Purification

Specifications

Specifications

BAC DNA, Plasmid DNA
BAC DNA, Plasmid DNA
  • 10mL Resuspension Buffer (R3)
  • 100 μl RNase A
  • 10mL Lysis Buffer (L7)
  • 10mL Precipitation Buffer (N3)
  • 50mL Equilibration Buffer (EQ1)
  • 125mL Wash Buffer (W8)
  • 25mL Elution Buffer (E4)
  • 15mL TE Buffer
  • 25 HiPure Columns
25 preps
Not High-throughput Compatible (Manual)
Room Temperature
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Bacterial Culture
Anion Exchange Resin
• 10 ml Resuspension Buffer (R3)
• 100 µl RNase A
• 10 ml Lysis Buffer (L7)
• 10 ml Precipitation Buffer (N3)
• 50 ml Equilibration Buffer (EQ1)
• 125 ml Wash Buffer (W8)
• 25 ml Elution Buffer (E4)
• 15 ml TE Buffer
• 25 HiPure Columns

Store all components at room temperature.
< 100 µg (Small-Scale) Plasmid DNA
PureLink™
Videos
Documents

For Research Use Only. Not for use in diagnostic procedures.