missing translation for 'onlineSavingsMsg'
Läs mer
Läs mer
Invitrogen™ T-REx™ Complete Kit, with pcDNA™4/TO© Vector
Klicka för att se tillgängliga alternativ
Kvantitet:
1 kit
Förpackningsstorlek:
5g
Beskrivning
Innehåller
20μg of pcDNA4/TO or 20μg each of pcDNA4/TO/myc-His A, B, and C; a positive control vector, pcDNA;6/TR, and forward and reverse sequencing primers plus 1g Zeocin (100mg/mL), 50mg of Blasticidin, and 5g of tetracycline. All vectors are supercoiled and provided lyophilized.
- Uses complete CMV promoter and adds control elements from bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of strongest mammalian promoter sequences known
- Specific Activation: uses a repressor mechanism that blocks transcription from powerful CMV promoter in absence of tetracycline
- Because elements do not use viral transactivators, you can achieve high-level expression from complete CMV promoter without secondary, non-specific activation of host genes
- T-REx transcriptional control elements are provided through two tetracycline operator sequences (TetO2) that have been inserted between TATA box of CMV promoter and transcriptional start site
- TetO2 sequence itself has no effect on expression
- When the tetracycline repressor protein (TR) is present, it effectively binds TetO2 sites and blocks transcription initiation
- Tetracycline added to culture medium binds to, and changes conformation of, TR protein; This change causes TR protein to release TetO2 sites, derepressing transcription from CMV promoter
- Result is high-level expression of gene of interest
- Expression levels can be modulated based on tetracycline concentration and can be induced to levels that are achieved with constitutive CMV expression vectors
- T-REx is a powerful inducible mammalian expression system that allows to regulate expression from complete human cytomegalovirus (CMV) enhancer-promoter
- Complete CMV enhancer-promoter sequence containing two copies of tetracycline operator TetO2 sequence for high-level regulated expression
- Zeocinor hygromycin resistance gene for effective selection of stable mammalian cell lines
- Large multiple cloning site to simplify cloning
- In addition, pcDNA™4/TO/myc-His offers a c-myc epitope for rapid detection of the recombinant protein with an Anti-myc Antibody and a polyhistidine (6xHis) sequence for simple purification of recombinant protein with nickel-chelating resin and detection with Anti- His(C-term) Antibody
- Regulatory vector, pcDNA™6/TR, is provided for high-level expression of the tetracycline repressor (TR) protein
- Expresses Blasticidin resistance gene for rapid selection of mammalian cell lines that stably express the TR protein
Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Regulated Expression
Specifikationer
Specifikationer
| Konstitutivt eller inducerbart system | Inducible |
| Leveranstyp | Transfection |
| Inducerande medel | Tetracycline |
| Promotor | CMV/TO |
| Produkttyp | T-Rex Complete Cloning Kit with Vector |
| Urvalsagent (eukaryot) | Zeocin™, Blasticidin |
| Innehåll och lagring | The T-REx™ Core Kit includes 20 μg of pcDNA™4/TO, a positive control vector, pcDNA™6/TR, and forward and reverse sequencing primers. The T-REx™ Complete Kit includes the Core Kit plus 1 g Zeocin™ (100 mg/ml), 50 mg of Blasticidin, and 5 g of tetracycline. All vectors are supercoiled and provided lyophilized. Store all components at -20°C. Zeocin™ and tetracycline should be protected from exposure to light. All components are guaranteed stable for 6 months when properly stored. |
| Protein Tag | Untagged |
| Kloningsmetod | Restriction Enzyme/MCS |
| Kvantitet | 1 kit |
| Visa mer |
For Research Use Only. Not for use in diagnostic procedures.
Korrigering av produktinnehåll
Din input är viktig för oss. Fyll i det här formuläret för att ge feedback relaterad till innehållet på denna produkt.
Produkttitel
Hittar du en möjlighet till förbättring?Dela en innehållskorrigering