missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10F' Chemically Competent E. coli
Click to view available options
No. of Reactions:
20 Reactions
40 Reactions
Unit Size:
Each
Description
The TA Cloning Kit uses the pCR2.1 cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.
- Fast and convenient: 15-minute, room-temperature ligation
- Efficient: blue/white screening and >80% clones with correct insert
- Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
- Hassle-free: eliminates any enzymatic modifications of PCR product
- Streamlined: does not require use of PCR primers that contain restriction sites
- 3'-T overhangs for direct ligation of Taq-amplified PCR products
- T7 promoter for in vitro RNA transcription and sequencing
- Versatile polylinker with flanking EcoR I sites for easy excision of inserts
- M13 forward and reverse primer sites for sequencing
- How TA Cloning™ Works
- Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to 3' ends of PCR products
- Llinearized vector supplied in this kit has single 3' deoxythymidine (T) residues
- Allows PCR inserts to ligate efficiently with vector
- Kit Configurations
- TA Cloning™ Kit is offered in a variety of configurations: without competent cells (K2020-20 and K2020-40), with One Shot™ INVF' Chemically Competent E. coli (K2000-01 and K2000-40), with One Shot™ TOP10F' Chemically Competent E. coli (K2030-01 and K2030-40), and with One Shot™ TOP10 Chemically Competent E. coli (K2040-01 and K2040-40) in 20- and 40-reaction kit sizes
Cloning, PCR Cloning
Specifications
Specifications
| Bacterial or Yeast Strain | TOP10F ́ |
| Cell Type | Chemically Competent E. coli |
| Cloning Method | TA Cloning |
| Content And Storage | TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
| Format | Kit |
| For Use With (Application) | PCR Cloning |
| No. of Reactions | 40 Reactions |
| Product Line | One Shot™ |
| Product Type | Cloning Kit |
| Promoter | T7 |
| Show More |
For Research Use Only. Not for use in diagnostic procedures.
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10F' Chemically Competent E. coli >
Spot an opportunity for improvement?Share a Content Correction