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Invitrogen™ Zero Blunt™ TOPO PCR Cloning Kit for Subcloning

Combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO vector to allow easy, high-efficiency cloning of blunt-end PCR products

Brand:  Invitrogen™ K283020

5120.25 SEK valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.07000kg

Product Code. 10434012

  • 7680.00 SEK / Each
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For Research Use Only. All usage must comply with product instructions.


Includes: Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid

Description

Description

  • Unique technology to minimize background
  • Get your clone: 95% efficiency
  • Multiple primer sites (T7, T3, M13F, M13R) make sequence analysis or PCR easy and convenient
  • Kanamycin and Zeocin resistance genes for your choice of selection
  • Convenient, validated restriction sites in MCS for further subcloning
  • Includes MachI™ T1R competent cells for fast miniprep and shortens cloning time

Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Cloning, Methylation Analysis, PCR Cloning, RNAi, Epigenetics and Non-Coding RNA Research, Sequencing Bisulfite-Treated DNA

Specifications

Specifications

Mach1™
Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Chromatin Biology
PCR Cloning Kit
Blunt DNA Cloning Vectors
Blunt TOPO™
Kit
One Shot™
25 Reactions
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For Research Use Only. Not for use in diagnostic procedures.