Tocris Bioscience™ Janelia Fluor 585, NHS ester

Key Information: Janelia Fluor™ 585, NHS ester is an orange fluorogenic fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging. Applications: Suitable for confocal microscopy, two-photon excitation fluorescence microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Cell permeable. Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag™ and SNAP-tag™. Excitation and emission maxima (λ) are 585 nm and 609 nm, respectively; quantum yield = 0.78; extinction coefficient = 156,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); A280 correction factor = 0.099; lactone-zwitterion equilibrium constant (KL-Z) < 0.0001. Exhibits low non-specific background staining. Please see the protocol for further information on protein/antibody labeling and conjugation. To measure the absorbance spectrum of this dye we recommend the following solvent: trifluoroethanol plus 0.1% TFA. We also offer Janelia Fluor™ conjugated antibodies and custom conjugation services with our sister company Novus Biologicals. HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc. Janelia Fluor is a registered trademark of Howard Hughes Medical Institute. This product has been recently renamed. The previous name for this product was Janelia Fluor™ 585, SE.