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Tocris Bioscience™ Janelia Fluor™ 646, free acid
Beskrivning
Key Information: Janelia Fluor™ 646, free acid is a red fluorogenic fluorescent dye; supplied with a free acid reactive group. Suitable for live cell imaging.Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor™ HaloTag™ and SNAP-Tag™ ligands, for use in live cell imaging experiments. Cell permeable.Properties and Photophysical Data: Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1. A280 correction factor = 0.19; lactone-zwitterion equilibrium constant (KL-Z) = 0.0012. To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA. We also offer Janelia Fluor™ conjugated antibodies and custom conjugation services with our sister company Novus Biologicals. HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc. Janelia Fluor is a registered trademark of Howard Hughes Medical Institute. This product will replace Janelia Fluor™ 646, free acid (Cat. No. 6993).
Specifikationer
Specifikationer
| Kemiskt namn eller material | 1-[7-(1-Azetidinyl)-10-(2,5-dicarboxyphenyl)-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium inner salt |
| CAS | 1811539-57-7 |
| Kvantitet | 5 mg |
| Mål | Free Acid Janelia Fluor Dyes |
| Molekylformel | C29H28N2O4Si |
| Renhet | 0.95 |
Produkttitel
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