missing translation for 'onlineSavingsMsg'
Läs mer
  1. Home
  2. Products
  3. Proteinanalysreagenser
  4. lysater
  5. Cell Line Lysates
  6. 16039495

JAR (human choriocarcinoma) Whole cell lysate, Denatured; Abnova

Produktkod. 16039495
Change view
Klicka för att se tillgängliga alternativ
Kvantitet:
200 μg
Förpackningsstorlek:
200µg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Produktkod. Kvantitet unitSize
16039495 200 μg 200µg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Denna artikel kan inte returneras. Se returpolicy
Produktkod. 16039495 Leverantör Abnova Leverantörsnummer L003V1.200ug

Vänligen för att beställa denna produkten. Behöver du ett Webbkonto? Registrera ditt konto idag!

Denna artikel kan inte returneras. Se returpolicy

Beskrivning

Western Blotting

Specifikationer

För användning med (applikation) Western Blot
Vävnad Placenta
Beskrivning Whole cell lysate (denatured)
Lysisbuffert Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Beredningsmetod Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Kvalitetskontrolltestning 12.5% SDS-PAGE Stained with Coomassie Blue.
Kvantitet 200 μg
Förvaringsbuffert In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Värd art Human
Koncentration 3 mg/mL
Innehåll och lagring Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Show More Show Less
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.