Ki-67, clone: 20Raj1, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5699-80
Additional Details : Weight : 0.01000kg
DescriptionDescription: The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms of Ki-67 exist, a 345 and 395kDa form that are expressed in dividing cells. Ki-67 is expressed in all cell types and is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but is absent from resting cells (G0). During interphase, Ki-67 expression is localized to the nucleus but redistributes to the chromosomes during mitosis and has specifically been found to associate with heterochromatin-bound proteins such as chromobox protein homolog 3 (CBX3). In studies of tumor cells, Ki-67 expression has been used as a marker for determining the fraction of proliferating cells within a given population of tumor cells.This monoclonal antibody 20Raj1 recognizes canine (dog) Ki-67.Applications Reported: This 20Raj1 antibody has been reported for use in immunocytochemistry and immunohistochemistry on formalin-fixed paraffin embedded tissues.Applications Tested: This 20Raj1 antibody has been tested by immunocytochemistry on paraformaldehyde fixed and permeabilized MCF7, MDCK, and MDA-MB-175 cell lines. This 20Raj1 antibody has been tested by immunohistochemistry on FFPE human skin using citrate buffer antigen retrieval. For immunocytochemistry and immunohistochemistry, this can be used at less than or equal to 0.5-1 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|antigen identified by monoclonal antibody Ki-67, antigen KI-67, KIA, proliferation-related Ki-67 antigen, protein phosphatase 1, regulatory subunit 105, RP11-380J17.2|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)|
|IgG1, kappa, kappa|
|Store at 2-8°C.|
For Research Use Only.