Ki-67 Rat anti-Canine, Cynomolgus Monkey, Human, Mouse, Non-human primate, Rat, Biotin, Clone: SolA15, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 13-5698-80
Additional Details : Weight : 0.09500kg
DescriptionPlease see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.125 µg per test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered. Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|antigen identified by monoclonal antibody Ki-67; antigen KI-67; KIA; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2|
|Canine, Cynomolgus Monkey, Human, Mouse, Non-human primate, Rat|
|Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)|
|4° C, store in dark, DO NOT FREEZE!|
|100686578, 102135895, 17345, 291234, 4288|
For Research Use Only.