Ki-67, FITC, clone: SolA15, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 11-5698-80
Additional Details : Weight : 0.25000kg
DescriptionDescription: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the Ki-67 labeling index refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin).The SolA15 antibody also recognizes human, non-human primate and canine Ki-67.Applications Reported: This SolA15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistochemical staining, and immunocytochemistry.Applications Tested: This SolA15 antibody has been tested by immunocytochemistry on fixed and permeabilized OLN93 cells at less than or equal to 10 μg/mL. It has also been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat 00-5523) and protocol. For flow application this can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|antigen identified by monoclonal antibody Ki-67, antigen KI-67, KIA, proliferation-related Ki-67 antigen, protein phosphatase 1, regulatory subunit 105, RP11-380J17.2|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.