Epredia™ Lab Vision™ UltraVision™ ONE Detection System: HRP Polymer/AEC Chromogen

UltraVision ONE is a robust ONE-step polymer system that provides increased sensitivity, time savings and detection simplicity. The UltraVision ONE HRP polymer is an innovative, patented technology. It consists of smaller amino acid polymer subunits that minimize conflicts in binding the target protein. Decreased binding conflicts result in more consistent staining and better signal amplification. Ultimately, this gives the user higher sensitivity and antibody efficiency. UltraVision ONE HRP polymer allows the use of less antibody to obtain better signal-to-noise ratios. This system is also biotin-free, which eliminates background staining found with traditional biotin-based detection methods. For optimal interpretation of results, appropriate positive and negative controls must be included.

• Specificity: anti-Mouse IgG (H+L), anti-Rabbit IgG (H+L)

• Enzyme: Peroxidase

• Chromogen/Substrate: 3-Amino-9-ethylcarbazole (AEC)

Includes:

TL-015-HAJ: 15mL Ultra V Block (TA-015-UB), 15mL Hydrogen Peroxide Block (TA-015-HP), 15mL UltraVision ONE HRP Polymer (TL-015-PHJ), 15mL AEC Single Solution (TA-015-SA)

Note:

UltraVision ONE HRP polymer detection system detects rabbit and mouse immunoglobulins bound to an antigen in tissue sections. The specific primary antibody is located by a universal secondary antibody polymer formulation. The amino acid polymer is conjugated to horseradish peroxidase and the Fab fragments of goat anti-rabbit and goat anti-mouse. The polymer complex is then visualized with an appropriate substrate/chromogen.