Invitrogen™ LAMP2 Polyclonal Antibody

Beskrivning

PA1-655 detects lysosome-associated membrane protein-2 (LAMP2) from rat, mouse and human tissues. PA1-655 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry. By Western blot, this antibody detects glycosylated and non-glycosylated LAMP-2, depending on the lysis buffer used. PA1-655 immunizing peptide corresponds to amino acid residues 400-411 from rat LAMP2. This sequence is completely conserved between rat, mouse, and guinea pig LAMP2. PA1-655 immunizing peptide (Cat. # PEP-039) is available for use in neutralization and control experiments.

LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is approximately 46 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of about 100-130 kDa. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. LAMP2 is used to monitor a very selective form of autophagy called chaperone-mediated autophagy. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. The LAMP protein has 3 variant forms: LAMP-2A, -2B, and 2C. LAMP2, together with CD107a/LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, and LAMP2 is an important regulator in successful phagosomal maturation. LAMP2 deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danon's disease).