missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ LanthaScreen™ TR-FRET ERR beta Coactivator Assay Kit, goat
LanthaScreen™ TR-FRET ERR beta Coactivator Assay Kit, goat
Brand: Invitrogen™ PV4800
Additional Details : Weight : 0.09000kg
Description
This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15139:The LanthaScreen™ TR-FRET ERR (estrogen related receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential ERR beta ligands as inverse agonists of ligand-dependent coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant ERR beta ligand-binding domain (ERR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.
To assay:
When running the LanthaScreen™ ERR beta TR-FRET Coactivator Assay, ERR beta-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound.
Based on the biology of the ERR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (see Figure 1).
Specifications
LanthaScreen™ TR-FRET ERR Beta Coactivator Assay Kit, 800 x 20μL Assays, 384-Well Plate Format, Tb (Terbium) Dye Type, Fluorescent Detection Method, TR-FRET Technique, For Pharma & Biopharma, Target & Lead Identification & Validation | |
Microplate reader | |
800 x 20 μL Assays | |
384-well Plate | |
Pharma and biopharma, drug discovery and development, target and lead identification and validation, nuclear receptor biology, biochemical nuclear receptor assays, nuclear receptor coregulator interaction |
Fluorescent | |
ERR beta-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-labeled anti-GST antibody, and buffers | |
For research use only (RUO) | |
-80°C/-20°C/4°C | |
LanthaScreen™ TR-FRET ERR Beta Coactivator Assay Kit |
For Research Use Only. Not for use in diagnostic procedures.