missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ LanthaScreen™ TR-FRET ERR beta Coactivator Assay Kit, goat

LanthaScreen™ TR-FRET ERR beta Coactivator Assay Kit, goat

Brand:  Invitrogen™ PV4800

Additional Details : Weight : 0.09000kg

Product Code. 10708775

  • 17380.00 SEK / Pack of 800
Estimated Shipment: 16-04-2024
to see stock.
Add to basket

For Research Use Only. All usage must comply with product instructions.


Description

Description

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15139:

The LanthaScreen™ TR-FRET ERR (estrogen related receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential ERR beta ligands as inverse agonists of ligand-dependent coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant ERR beta ligand-binding domain (ERR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:

When running the LanthaScreen™ ERR beta TR-FRET Coactivator Assay, ERR beta-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound.
Based on the biology of the ERR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (see Figure 1).
Specifications

Specifications

LanthaScreen™ TR-FRET ERR Beta Coactivator Assay Kit, 800 x 20μL Assays, 384-Well Plate Format, Tb (Terbium) Dye Type, Fluorescent Detection Method, TR-FRET Technique, For Pharma & Biopharma, Target & Lead Identification & Validation
Microplate reader
800 x 20 μL Assays
384-well Plate
Pharma and biopharma, drug discovery and development, target and lead identification and validation, nuclear receptor biology, biochemical nuclear receptor assays, nuclear receptor coregulator interaction
Fluorescent
ERR beta-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-labeled anti-GST antibody, and buffers
For research use only (RUO)
-80°C/-20°C/4°C
LanthaScreen™ TR-FRET ERR Beta Coactivator Assay Kit
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.